The medical industry now uses drugs that can be derived from different
herbal plants which are useful for the treatment of human diseases. There are
proven scientific tests that medicinal plants used by folk as remedies was
proven to be effective, especially in the field of fungal diseases. Fungal
infections are difficult to treat. Treatment involves topical (applied to the skin or
nail) or oral (swallowed) medications called antifungals. The type of antifungal
used depends on the specific type of fungus that is causing the infection. These
fungus can cause serious disease and nowadays is a major people health
problem (Swierzewski, 2015).
This study was carried out to determine the Antibacterial Efficacy of
Silver Nanoparticles from Takip Kuhol (Centella asiatica) Leaf Extract. The
antifungal efficacy of silver nanoparticles from takip kuhol leaf extract was assessed by determining the characteristics of silver nanoparticles in terms of
SEM Analysis and Morphological Examination, and the level of antifungal
efficacy of silver nanoparticles from takip kuhol leaf extract. The concentration
of 100% of the silver nanoparticles from takip kuhol leaf extract were tested
against gram negative fungi Trichophyton mentagrophytes and gram positive
fungi Aspergillus niger.
SEM Analysis revealed the different sizes of silver nanoparticles from
takip kuhol leaf extract. Each silver nanoparticles have a size less than 100 nm
and circular in shape. The average size of silver nanoparticles from takip kuhol
leaf extract was 36. 64 nm less than 100 nm, which is an acceptable size of
silver nanoparticles. The result shows that the obtained product is comparable
and acceptable in terms of size and shape to a standard silver nanoparticle.
Morphological examination showed that biosynthesizing silver
nanoparticles from takip kuhol leaf extract produced a circular silver
nanoparticle with an average size of 36.64 nm changing the color of the silver
nitrate solution from clear to yellowish grey.
Antifungal assay revealed that no antifungal efficacy of sliver
nanoparticles from takip kuhol leaf extract by the area of inhibition on the gram
negative fungi Trichophyton mentagrophytes and gram positive fungi
Aspergilus niger using 100% concentration.

The result on the gram negative fungi T. mentagrophytes shows that no
clearing zone and antimicrobial index was achieved in Treatment 4 or 100%
concentration of silver nanoparticles which is equal to 0 nm. On the other hand,
also the gram positive fungi A. niger, Treatment 4 or 100% concentration of
silver nanoparticles shows no clearing zone and antimicrobial index which is
equal to 0 nm.
From the result shows that in 100% concentration, of silver
nanoparticles from takip kuhol leaf extract has no antifungal efficacy against
both gram negative fungi Trichophyton mentagrophytes and gram positive fungi
Aspergillus niger.
The result of One-Way ANOVA in terms of effectiveness of the
treatment 1 between the gram negative fungi Trichophyton mentagrophytes
and gram positive fungi Aspergillus niger shows that the computed F-value is
0.28999 and the critical value is 18.51282. Since the computed F value,
0.28999 is less than the computed critical value, 18.51282; therefore, the nul
hypothesis is accepted. There is no significant difference in the antifungal
efficacy of one concentration of silver nanoparticles from Takip Kuhol Leaf
Extract between the two types of fungi. From the result, it only shows that the
antifungal efficacy of silver nanoparticles from takip kuhol leaf extract shows no
significant difference of effectiveness for both gram negative fungi Trichophyton mentagrophytes and gram positive fungi Aspergillus niger given at a higher
concentration.
Takip Kuhol (Centella asiatica) Leaf Extract revealed as not a potential
biological antifungal agent against both gram negative fungi Trichophyton
mentagrophytes and gram positive fungi Aspergillus niger given at a higher
concentration.